Dmitry Kolpashchikov, UCF
Event Date:
October 26, 2017 – 3:30 PM
Location:
Burson 115
Event Date:
October 26, 2017 – 3:30 PM
Location:
Burson 115
Ph.D Nanoscale Science Seminar Series | Fall 2017
Dr. Dmitry M. Kolpashchikov
University of Central Florida
Associate Professor, Department of Chemistry
will be presenting
“From Hybridization Probes to DNA Nanorobots”
Abstract:
Hybridization of oligonucleotide probes has been used in a number of bioanalytical assays as well as proposed for therapy in such formats as antisense and RNA interference. To overcome common limitations of conventional hybridization approaches we construct sophisticated multifunctional DNA-based systems, which we called ‘DNA machines’. This presentation will highlight the evolution of hybridization probes to DNA machines that can simultaneously accomplish the following functions: (i) tightly bind natural RNA and unwind its secondary structure, (ii) recognize targeted RNA fragment with high selectivity, (iii) produce fluorescent signal and (iv) catalytically amplify the signal. Possible applications of the DNA machines in cancer therapy as well as perspectives of their upgrade to DNA nanorobots will be discussed.
References
- Cox A. J., Bengtson, H. N., Rohde K. H., Kolpashchikov D. M. (2016) DNA Nanotechnology for Nucleic Acid Analysis: Multifunctional Molecular DNA Machine for RNA Detection. ChemComm, 52, 14318-14321.
- 2.Bakshi S.F., Guz N., Zakharchenko A., Deng H., Tumanov A., Woodworth C. D., Minko S., Kolpashchikov D. M., Katz E. (2017) Magnetic Field-Activated Sensing of mRNA in Living Cells. Journal of the American Chemical Society 139, 12117-12120.
Biography:
Dmitry M. Kolpashchikov received his PhD in Bioorganic Chemistry from Novosibirsk Institute of Bioorganic Chemistry in 1999. He then studied influenza virus RNA polymerase at the National Institute of Genetics, Japan. In 2002 he joined the research group of Dr. Milan Stojanovic at Columbia University, where he worked on the development of biosensors and molecular computers. He is currently an Associate Professor in the Department of Chemistry at the University of Central Florida. His research interests include development of nucleic acid DNA-based sensors and molecular machines.