Education:

B.S.: Ball State University, Muncie, Indiana
Ph.D.: Purdue University, West Lafayette, Indiana.
Post-doc: Purdue University, West Lafayette, Indiana

Field of Interest: Biochemistry

Research Focus:

1. Studies of Glycosylasparaginase, the Enzyme Involved in the Most Common Disorder of Glycoprotein Degradation The catabolism of glycoproteins to the constituent amino acids and monosaccharides involves many different enzymes. While the enzymes that hydrolyze the peptide bonds in the polypeptide chain to amino acids show a generally broad activity toward different amino acid side chains, the enzymes that hydrolyze the bonds between the sugars in the carbohydrate moieties of glycoproteins generally have a high degree of specificity. A key enzyme in the catabolism of N-linked carbohydrate moieties is glycosylasparaginase, which hydrolyzes the amide bond between asparagine and N-acetylglucosamine to give aspartic acid and 2-acetamido-2-deoxy-b-D-glucopyranosylamine. A decrease in activity of this enzyme gives rise to aspartylglycosaminuria, an inherited lysosomal storage disease, that leads to mental retardation and a shortened life span as the metabolite accumulates in cells, tissues, and body fluids. This disease has recently been recognized as the most common disorder of glycoprotein metabolism. There is no cure for the disorder. Mutations that give rise to the disorder have been elucidated and a crystal structure for the enzyme has been published. My lab is studying the fundamental physical and kinetic properties of the enzyme. We synthesize potential substrate analogues for the enzyme, inhibitors, possible suicide substrates, and transition-state analogues, and study their properties with the enzyme. We are also using molecular modeling of the enzyme in order to study various properties.
2. The 18O Isotope Shift in NMR. NMR spectroscopy is a very important analytical tool in chemistry. It is used in almost all areas of chemistry, including analytical, biochemistry, inorganic, organic, and physical chemistry. One small area, and specialization, of study in NMR is the effect of isotopes on NMR active nuclei. Oxygen has three naturally-occurring isotopes, 16O, 17O and 18O; 16O is the most abundant at 99+%. Two NMR-active nuclei that are in important oxygen-containing compounds are 13C and 31P. The NMR signals of 13C and 31P have slightly different chemical shifts when bonded to 16O and 18O; the differences are very small – a few ppb (parts per billion) – but can be readily detected when the NMR spectrometer is correctly set up. We are using these 18O isotope shifts in 13C NMR and 31P NMR to study reactions and properties of molecules.

Risley Group Home Page

Education:

B.S.: Otterbein College
M.S.: University of Arizona
Ph.D.: University of Arizona
Post-doc: University of Lausanne, Lausanne, Switzerland

Field of Interest: Organic Chemistry

Research Focus:

My research has centered on the preparation, reaction and structure of carbanionic species. We are currently preparing organometallic reagents as chiral auxiliaries for organic synthesis. We are preparing functional monomers for preparing functional polymers. We are using the rapid injection NMR technique to help understand the mechanisms for organometallic conjugate addition reactions.

Ogle Group Home Page

Field of Interest: Physical and Analytical Chemistry

Research Focus:

My research interests are related to the investigation of dynamics using nuclear magnetic resonance (NMR) spectroscopy. Past work includes the study of molecular rotation in crystalline solids, chain dynamics in amorphous polymers, and rate studies of exchanging spin systems. Current interests include reaction kinetics of anionic polymerizations and structural determinations of reaction intermediates in organocuprate addition reactions.

Dr. Michael Murphy’s site

Field of Interest: Physical Chemistry

Research Focus:

X-ray Crystallography: Determination of molecular structures by X-ray crystallographic methods. The technique of single-crystal X-ray crystallography can be used to determine the detailed molecular structure of chemical compounds. Because this is a completely general method, it can be applied to almost any compound of chemical interest, and is thus an important tool in many different areas of research. The determination of a substance’s structure by X-ray methods involves several steps, including 1) preparation of suitable crystals for study, 2) preliminary X-ray investigation for the determination of crystal quality and lattice type, 3) collection of high accuracy intensity data on an automated X-ray diffractometer, and 4) reduction and analysis of the data utilizing high-speed computers. Structure determinations are carried out on compounds of interest in a variety of research endeavors; the particular compounds studied often depend on the immediate research interests of faculty colleagues. Compounds recently studied include templates for the synthesis of chiral organic compounds, and both mononuclear and polymeric transition metal complexes.

Jones Research Home Page

Field of Interest: Chemical Instrumentation Specialist

Dr. Clifford Carlin’s site

Field of Interest: Organic Chemistry

Field of Interest: Organic Chemistry

Field of Interest: Physical Chemistry

Field of Interest: Inorganic Chemistry

Research Focus:

Computational chemistry, computational materials and Supercomputing. – Lewis acid-base reaction chemistry and inorganic cluster compounds. – Photopolymers, photochemistry and lithography. – Materials processing and plasma chemistry. – Inorganic polymers and materials having unusual electronic properties. – Homogeneous and heterogeneous transition metal catalysts. – Microelectronic and Micromechanical Systems.

DuBois Group Home Page